Fe,Mn,Zn等典型性微量营养元素对藻类增殖的影响是探讨藻华成因分析的重要内容．通过设计系列正交实验，研究代表性微量元素Fe,Mn,Zn对常见藻华生物(蓝藻、绿藻)的影响，结果表明：P,Fe,Mn,Zn的浓度变化对藻生长速率激励效果明显，显著性顺序依次为：ZnPFeMn．通过单独添加Fe,Mn,Zn的方法进一步进行了藻增长潜力实验．结果表明：在Fe,Mn,Zn浓度分别为0.20mg/L, 0.13mg/L, 0.033mg/L时，藻类生长速率达到最大值，低于该浓度时，金属离子浓度与藻类生长速率、生物量呈正相关；高于该浓度时，金属离子浓度与藻生长水平呈反相关；拟合Fe,Mn,Zn浓度与藻生长速率之间的数值关系，分别得到相关系数为0.93, 0.87, 0.90的回归方程；根据微量元素与藻类生长速率之间的相关性，可以对藻华的爆发时间或程度进行一定精度的预测．正交实验中，锰的激励作用受微量元素之间的竞争性抑制而被消弱，反映出锰的作用机理比较复杂，有待于深入研究．
The effect of micro-nutrients (Fe, Mn, Zn) on the proliferation of planktonic algae colony is one of the most important contents in the researches of cause analysis of algae blooms. Experiments on the impacts of typical micro-nutrients (P, Fe, Mn, Zn) on common algal blooms organisms (cyanobacteria, chlorophyta) were carried out by series of orthogonal experiments. The results show that these nutrients have significant impact on algae growth and stimulated cell-specific growth rates with ZincPhosphorusIronManganese. AGP (algae growth potential) experiments by separate addition of iron, manganese and zinc were further performed through single-factor experiments. The data obtained show that maximum algae growth rate appeared when concentration of Fe,Mn,Zn were 0.20mg/L, 0.13mg/L, 0.033mg/L,respectively. Furthermore, a positive motivation to the growth rate and biomass of algae was emerged with the concentrations of Fe, Mn and Zn range from 0.01~0.20, 0.01~0.13, 0.001~0.033mg/L, while there is a negative motivation with the increase of the concentrations of Fe, Mn and Zn above 0.20, 0.13, 0.033mg/L, respectively. By fitting the numerical relationships between concentrations of Fe, Mn, Zn and algal cell-specific growth rate, regression equations with correlation coefficient of 0.93, 0.87, 0.90 were obtained respectively. The results make it be able to predict the time and severity of outbreak of algae blooming to a certain precision extent according to the correlation between trace elements and algae growth rate or biomass. Experiment consequence also suggested that the significant levels of Manganese to algae had been severely weakened by the inhibitory effect among multi-microelements during orthogonal experiments. These results reveal that the mechanism of Manganese is rather complicated that further researches are needed to be performed.