In order to investigate phenolics and their antioxidant activities of litchi pulp, the paper used 60% ethanol and 80% acetone to extract polyphenol from litchi chinensis pulp separately. The polyphenols were separated according to the polarity separation size, which was combined with DPPH, FRAP and ABTS to evaluate antioxidant activity of different polarity polyphenols. The results showed that antioxidant activity of the litchi pulp polyphenol extract by 80% acetone extract was significantly stronger than the 60% ethanol（P0.05）. Polyphenol content of different polarity were significantly different (P0.05). The water layer had the highest polyphenol content, which was followed by the n-butyl alcohol part. The antioxidant activity of different polar solvent polyphenols were significantly different (P0.05), which the ethyl acetate showed the highest antioxidant activity , followed by n-butyl alcohol part. The activity of acetone extract polyphenol was significantly higher than the ethanol extract of polyphenols (P0.05). Through the effective separation of different polarity polyphenols and evaluation of antioxidant activity, the study formed the foundation for the research of the highly active polyphenols of litchi pulp.