The Effect of Accumulated Exercise on Myocardial Collagen Fibers in Insulin Resistant Mice
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摘要: 累积运动是打破久坐行为和改善代谢健康的有效手段。为了探究累积运动的健康干预价值，文章通过对胰岛素抵抗(IR)小鼠进行长期的运动干预，比较了不同强度的累积运动和持续运动对小鼠IR状态、心肌胶原纤维和心肌纤维化蛋白表达的影响。首先，高脂饲料喂养112只C57BL/6J雄性小鼠(4周龄)诱导IR小鼠模型；然后，用中等强度持续运动、中等强度累积运动和大强度累积运动干预IR小鼠8周，进行口服葡萄糖耐量实验(OGTT)，眼眶取血后处死小鼠, 摘取其心脏组织；其次，使用ELISA方法检测小鼠血清空腹胰岛素(FINS)水平和心肌组织Ⅰ型胶原(COLⅠ)、Ⅲ型胶原(COLⅢ)、平滑肌肌动蛋白(α-SMA)含量；使用Western blot方法检测小鼠心肌组织转化生长因子β1(TGF-β1)、Smad3、结缔组织生长因子(CTGF)蛋白表达水平。结果显示：3种运动均可以改善IR小鼠的IR状态和心肌纤维化程度，大强度累积运动与中等强度持续运动的作用效果相当，中等强度累积运动的作用效果较弱。
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关键词:
- 累积运动 /
- 胰岛素抵抗 /
- 心肌胶原纤维 /
- TGF-β1/Smad3
Abstract: Accumulated exercise is an effective means for breaking sedentary behavior and improving metabolic health. To explore the health intervention value of accumulated exercise, the effects of different intensities of accumulated exercise and continuous exercise have been compared on insulin resistance (IR) status, myocardial collagen fibers, and myocardial fibrosis protein expression in mice through long-term exercise. The IR mouse model was induced by feeding 112 C57BL/6J male mice (4 weeks old) with high-fat diet. The IR model mice were intervened with moderate intensity continuous exercise, moderate intensity accumulated exercise and high intensity accumulated exercise for 8 weeks. Oral glucose tolerance test(OGTT) was performed, and the mice were sacrificed after blood was collected from the orbit to extract heart tissue. The ELISA method was used to detect serum Fasting insulin(FINS) level, myocardial tissue collagen Ⅰ(COLⅠ), collagen Ⅲ(COLⅢ) and α-smooth muscle actin (α-SMA) content. The Western blot method was used to detect mouse myocardial tissue transforming growth factor-β1(TGF-β1), Smad3 and connective tissue growth factor(CTGF) protein expression level. The results showed that all three kinds of exercise could improve the IR state and myocardial fibrosis degree of IR mice, the effects of high intensity accumulated exercise and moderate intensity continuous exercise were equivalent, while the effect of mode-rate intensity accumulated exercise was weaker.-
Key words:
- accumulated exercise /
- insulin resistance /
- myocardial collagen fibers /
- TGF-β1/Smad3
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图 1 高脂饲料喂养对C57BL/6J小鼠OGTT的影响
Figure 1. Influence of OGTT by high-fat diet in C57BL/6J mice
图 2 各组小鼠心肌组织TGF-β1、Smad3、CTGF蛋白的相对表达量
Figure 2. Relative expression of TGF-β1, Smad3 and CTGF protein in myocardial tissue of each group
表 1 小鼠运动干预方案
Table 1. The program of exercise intervention for mice
分组 运动速度/(m·min-1) 单次运动时间/min 重复次数 次间间隔时间/h MCE 11 50.0 1 / MAE 11 12.5 4 3 HAE 19 7.5 4 3 
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表 2 普通饲料或高脂饲料喂养后小鼠的BW和IR评价结果
Table 2. Mice's BW and IR model results after dietary intervention
分组 BW/g OGTT-AUC FPG/(mmol·L-1) FINS/(mU·L-1) QUICKI HOMA-IR NC 26.08±1.54 15.53±1.52 5.53±0.63 7.69±0.57 0.59±0.01 2.15±0.24 HFD 31.98±2.08** 23.13±2.11** 8.08±0.84** 10.39±1.44* 0.51±0.01** 4.00±0.26** 注：与NC组相比，*表示P<0.05，**表示P<0.01。
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表 3 运动干预后各组小鼠的BW、FPG、OGTT-AUC和FINS
Table 3. The BW, FPG, OGTT-AUC and FINS in mice after exercise
分组 BW/g FPG/(mmol·L-1) OGTT-AUC FINS/(mU·L-1) IRC 31.45±2.37 8.43±0.84 19.37±1.56 9.66±1.37 MCE 28.69±2.01## 6.82±0.25## 15.23±1.01## 7.58±0.67## MAE 27.71±1.99## 6.73±0.15## 14.10±0.92## 7.70±0.13# HAE 26.24±1.54##†† 6.47±0.29## 15.28±0.60## 7.44±0.45## 注：与IRC组相比：#表示P<0.05，##表示P<0.01；与MCE组相比：††表示P<0.01。
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表 4 运动干预后各组小鼠心肌组织胶原纤维含量
Table 4. The content of myocardial collagen fibers in mice after exercise
分组 COLI/(μg·L-1) COLIII/(μg·L-1) COLI/COLIII IRC 23.19±0.39 9.55±0.44 2.32±0.14 MCE 18.62±0.88## 7.98±0.40## 2.07±0.02# MAE 20.54±0.39##† 7.97±0.37## 2.29±0.12† HAE 19.18±0.61## 7.70±0.24## 2.20±0.02 注：与IRC组相比：#表示P<0.05，##表示P<0.01；与MCE组相比：†表示P<0.05。
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