Abstract: Glutathione peroxidase 4(GPX4) is a selenoprotein that can specifically catalyze the conversion of glutathione from lipid peroxides to lipid alcohols. Up-regulation of GPX4 expression plays an important role in inhibiting ferroptosis and related inflammation. In order to screen out the potential active small molecules that can activate glutathione peroxidase 4(GPX4) from Artemisia annua, 126 active small molecules of Artemisia annua were firstly retrieved from the Traditional Chinese Medicine System Pharmacology Database and Analysis Platform and 50 active small molecules of Artemisia annua were screened out according to drug-like properties. Further, 8 active small molecules of Artemisia annua were screened out through GPX4-ligand docking simulation and GPX4-ligand inte-raction pattern. Analysis and screening with GPX4-ligand free binding energy calculation found that artemisinin, patuletin and kaempferol acted similarly to the positive control 1d4(PKUMDL-LC-101-D03). Finally, molecular dynamics simulation was performed. The results showed that artemisinin, patuletin, kaempferol and the positive control 1d4 can form a stable complex with GPX4.