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拟南芥BLI基因调控植物衰老的研究

高璐 黄瑞华 张盛春

高璐, 黄瑞华, 张盛春. 拟南芥BLI基因调控植物衰老的研究[J]. 华南师范大学学报(自然科学版), 2021, 53(4): 49-54. doi: 10.6054/j.jscnun.2021057
引用本文: 高璐, 黄瑞华, 张盛春. 拟南芥BLI基因调控植物衰老的研究[J]. 华南师范大学学报(自然科学版), 2021, 53(4): 49-54. doi: 10.6054/j.jscnun.2021057
GAO Lu, HUANG Ruihua, ZHANG Shengchun. Regulation of Plant Senescence by BLI in Arabidopsis thaliana[J]. Journal of South China normal University (Natural Science Edition), 2021, 53(4): 49-54. doi: 10.6054/j.jscnun.2021057
Citation: GAO Lu, HUANG Ruihua, ZHANG Shengchun. Regulation of Plant Senescence by BLI in Arabidopsis thaliana[J]. Journal of South China normal University (Natural Science Edition), 2021, 53(4): 49-54. doi: 10.6054/j.jscnun.2021057

拟南芥BLI基因调控植物衰老的研究

doi: 10.6054/j.jscnun.2021057
基金项目: 

国家自然科学基金项目 31870301

详细信息
    通讯作者:

    张盛春,Email: sczhang@scnu.edu.cn

  • 中图分类号: Q37

Regulation of Plant Senescence by BLI in Arabidopsis thaliana

  • 摘要: BLISTER(BLI)蛋白是多种应激反应的调节因子,可以促进植物对环境的适应性. BLI蛋白通过抑制IRE1蛋白以维持植物的正常生长,缺失BLI蛋白的拟南芥植株出现多种发育缺陷表型. 文章以拟南芥bli突变体为实验材料,通过细胞生物学及遗传学方法检测其衰老相关表征,发现bli突变体植株早期出现叶片黄化、叶绿素含量下降、离子渗透率升高等一系列衰老表型;与此同时,bli突变体体内衰老相关基因表达上调,光合作用相关基因表达下调,表明BLI蛋白参与植物衰老过程;外源施加ROS导致野生型Col-0植株中BLI基因表达量减少; GUS显色结果表明BLI在植株各组织器官中均有表达,为进一步解析BLI调控衰老的分子网络提供了思路.
  • 图  1  不同BLI基因型植株的叶片表型

    Figure  1.  The leaf phenotypes of different BLI genotypes

    图  2  不同BLI基因型叶绿素相对含量及离子渗透率

    注:**表示相对于Col-0的显著性差异,P < 0.01.

    Figure  2.  The relative chlorophyll content and ionic permeability of different BLI genotypes

    图  3  BLI基因的组织表达模式分析

    Figure  3.  The patterns of BLI gene expression in the various tissues

    图  4  BLI与ROS的关系分析

    Figure  4.  The relationship between BLI and ROS

    图  5  BLI植株衰老相关基因表达分析

    Figure  5.  The expression of senescence-related genes in BLI plants

    表  1  引物对及序列

    Table  1.   Primer pairs and sequences

    引物名称 序列(5'-3')
    Actin-F CTACGAGCAGGAACTCGAGA
    Actin-R GATGGACCTGACTCGTCATAC
    BLI-F CAACCTGAGCAATCTCTTCAG
    BLI-R TAGTCTGTTGTATTTTTTGAAAAAT
    SAG12-F AAGACCAATCCAAAAGCAAC
    SAG12-R AATCAAAACCACCTCCTTCA
    SAG13-F TCAAGCCGACCACAGAG
    SAG13-R GCAGAGGATATGAGCACGA
    SAG101-F CTCTCTTCACACACCTGCTTT
    SAG101-R AGATGCAACAGACCCTCCT
    LHCA1-F GTTAAGGCTCAGGAATGGG
    LHCA1-R TGGCTAAGAACTCAATGGC
    RBCS-F TCCTAATTCTTGCCTTTGGT
    RBCS-R TTTGCTATGCTTTCAAGTGC
    CAB1-F TCACTGGTAAGGGACCGA
    CAB1-R CGAAGCAAAGACTGAAGCA
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-10-31
  • 网络出版日期:  2021-09-03
  • 刊出日期:  2021-08-25

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