Regulation of Plant Senescence by BLI in Arabidopsis thaliana
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摘要: BLISTER(BLI)蛋白是多种应激反应的调节因子，可以促进植物对环境的适应性. BLI蛋白通过抑制IRE1蛋白以维持植物的正常生长，缺失BLI蛋白的拟南芥植株出现多种发育缺陷表型. 文章以拟南芥bli突变体为实验材料，通过细胞生物学及遗传学方法检测其衰老相关表征，发现bli突变体植株早期出现叶片黄化、叶绿素含量下降、离子渗透率升高等一系列衰老表型；与此同时，bli突变体体内衰老相关基因表达上调，光合作用相关基因表达下调，表明BLI蛋白参与植物衰老过程；外源施加ROS导致野生型Col-0植株中BLI基因表达量减少; GUS显色结果表明BLI在植株各组织器官中均有表达，为进一步解析BLI调控衰老的分子网络提供了思路.Abstract: As a regulator of various stress responses, protein BLI promotes the adaptability of plants to the environment and maintains the normal growth of plants by inhibiting protein IRE1. Arabidopsis thaliana individuals without protein BLI are shown to have the mutant phenotype of pleiotropic development. The senescence-related characteristics of BLI mutant Arabidopsis thaliana plants were studied with the cell biology and genetic methods. It was found that a series of senescence phenotypes, such as leaf yellowing, decreased chlorophyll content and increased cell conductivity, appeared in the early stage of the BLI mutant plants and, at the same time, the expression of senescence-related genes in BLI mutant plants was up-regulated and the expression of photosynthesis-related genes was down-regulated. These results preliminarily proved that protein BLI was involved in plant senescence. Applying exogenous ROS to wild-type Col-0 plants showed that the BLI expression was reduced and GUS staining showed that BLI was expressed in all parts of the plant, which provides clues for further analyzing the molecular network for BLI regulating senescence.
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Key words:
- BLI /
- senescence /
- Arabidopsis thaliana
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图 2 不同BLI基因型叶绿素相对含量及离子渗透率
注：**表示相对于Col-0的显著性差异，P < 0.01.
Figure 2. The relative chlorophyll content and ionic permeability of different BLI genotypes
图 3 BLI基因的组织表达模式分析
Figure 3. The patterns of BLI gene expression in the various tissues
图 5 BLI植株衰老相关基因表达分析
Figure 5. The expression of senescence-related genes in BLI plants
表 1 引物对及序列
Table 1. Primer pairs and sequences
引物名称 序列(5'-3') Actin-F CTACGAGCAGGAACTCGAGA Actin-R GATGGACCTGACTCGTCATAC BLI-F CAACCTGAGCAATCTCTTCAG BLI-R TAGTCTGTTGTATTTTTTGAAAAAT SAG12-F AAGACCAATCCAAAAGCAAC SAG12-R AATCAAAACCACCTCCTTCA SAG13-F TCAAGCCGACCACAGAG SAG13-R GCAGAGGATATGAGCACGA SAG101-F CTCTCTTCACACACCTGCTTT SAG101-R AGATGCAACAGACCCTCCT LHCA1-F GTTAAGGCTCAGGAATGGG LHCA1-R TGGCTAAGAACTCAATGGC RBCS-F TCCTAATTCTTGCCTTTGGT RBCS-R TTTGCTATGCTTTCAAGTGC CAB1-F TCACTGGTAAGGGACCGA CAB1-R CGAAGCAAAGACTGAAGCA 
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[1] SCHUBERT V. SMC proteins and their multiple functions in higher plants[J]. Genome Research, 2009, 124: 202-214. http://europepmc.org/abstract/MED/19556774 [2] SCHATLOWSKI N, STAHL Y, HOHENSTATT M L, et al. The CURLY LEAF interacting protein BLISTER controls expression of polycomb-group target genes and cellular differentiation of Arabidopsis thaliana[J]. The Plant Cell, 2010, 22(7): 2291-2305. doi: 10.1105/tpc.109.073403 [3] JULIA A, SCHATLOWSKI N, DAVID H, et al. BLISTER regulates polycomb-target genes, represses stress-regulated genes and promotes stress responses in Arabidopsis thaliana[J]. Frontiers in Plant Science, 2017, 8: 1530/1-14. http://europepmc.org/abstract/MED/28955347 [4] LIU J X, HOWELL S H. Endoplasmic reticulum protein quality control and its relationship to environmental stress responses in plants[J]. The Plant Cell, 2010, 22(9): 2930-2942. doi: 10.1105/tpc.110.078154 [5] HONG Z H, QING T, SCHUBERT D, et al. BLISTER-regulated vegetative growth is dependent on the protein kinase domain of ER stress modulator IRE1A in Arabidopsis thaliana[J]. PLoS Genetics, 2019, 15(12): e1008563/1-19. http://www.ncbi.nlm.nih.gov/pubmed/31869326 [6] KLIMEŠOVÁ J, NOBIS M P, HERBEN T. Senescence, ageing and death of the whole plant: morphological prerequisites and constraints of plant immortality[J]. The New Phytologist, 2015, 206(1): 14-18. doi: 10.1111/nph.13160 [7] LIM P, NAM H. The molecular and genetic control of leaf senescence and longevity in Arabidopsis[J]. Current Topics in Developmental Biology, 2005, 67: 49-83. http://www.sciencedirect.com/science/article/pii/S0070215305670020 [8] VICKY B. The molecular biology of leaf senescence[J]. Journal of Experimental Botany, 1997, 48(2): 181-199. doi: 10.1093/jxb/48.2.181 [9] PYUNG L, HYE R, HONG G. Molecular genetics of leaf senescence in Arabidopsis[J]. Trends in Plant Science, 2003, 8(6): 272-278. doi: 10.1016/S1360-1385(03)00103-1 [10] PYUNG O, HYO J, HONG G N. Leaf senescence[J]. Annual Review of Plant Biology, 2007, 58: 115-136. doi: 10.1146/annurev.arplant.57.032905.105316 [11] 刘梦灵, 陈颖华, 张盛春. 过氧化氢对拟南芥生长素信号转导相关蛋白的影响[J]. 华南师范大学学报(自然科学版), 2017, 49(5): 64-71. http://journal-n.scnu.edu.cn/article/id/4155LIU M L, CHEN Y H, ZHANG S C. Effects of hydrogen peroxide on auxin signal associated proteins in Arabidopsis thaliana[J]. Journal of South China Normal University(Natural Science Edition), 2017, 49(5): 64-71. http://journal-n.scnu.edu.cn/article/id/4155 [12] 初梦圆, 于延冲. 影响植物叶片衰老因素的研究进展[J]. 生命科学, 2019, 31(2): 178-184. https://www.cnki.com.cn/Article/CJFDTOTAL-SMKX201902010.htmCHU M Y, YU Y C. The research progress of factors affecting plant leaf senescence[J]. Chinese Bulletin of Life Sciences, 2019, 31(2): 178-184. https://www.cnki.com.cn/Article/CJFDTOTAL-SMKX201902010.htm [13] 黎家, 李传友. 新中国成立70年来植物激素研究进展[J]. 中国科学: 生命科学, 2019, 49(10): 1227-1281. https://www.cnki.com.cn/Article/CJFDTOTAL-JCXK201910004.htmLI J, LI C Y. Seventy-year major research progress in plant hormones by Chinese scholars (in Chinese)[J]. Sciencia Sinica Vitae, 2019, 49: 1227-1281. https://www.cnki.com.cn/Article/CJFDTOTAL-JCXK201910004.htm [14] 王凯. 拟南芥中ABA调控自噬的机制的研究[D]. 重庆: 重庆大学, 2017.WANG K. ABA regulation of autophagy in Arabidopsis[D]. Chongqing: Chongqing University, 2017. -
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