Abstract: Conghua District, Guangzhou City, is chosen as the area for study, and the habitats of keystone species in this area are selected to be the ecological sources. The minimum cumulative resistance model is used to pick biological corridors and determine the ecological nodes, and the spatial ecological network is constructed on the basis of the greenways in Conghua District to enhance the regional ecological protection function and the spatial connectivity of the fragmentized ecological environments. The following results are obtained. First, the number of wild animal habitats of 300-500 hm² is the largest and these sources are heavily concentrated in the north with small landscape resistance. On the other hand, wild animal habitats are distributed between towns in the south with high landscape resistance. Second, the longer the corridors and the higher their resistance, the smaller their number. Third, the spatial ecological network enhances the ecological functions of the greenway system through the intersection between corridors, nodes and established greenways. The network can minimize the destruction to nature and enhance the connectivity between different landscapes and greenways so as to satisfy the need of the residents to take exercise and have a rest and the need of wildlife to spread and migrate.