水稻C2H2型锌指蛋白OsZAT12转化拟南芥功能的初步研究

A Preliminary Study of OsZAT12 with C2H2-Type Zinc Finger Transforming Arabidopsis

  • 摘要: 摘要:植物C2H2型锌指蛋白是植物转录调节因子中的大家族,通过转录调控、翻译后调节和蛋白质间的相互作用在植物发育和环境胁迫中发挥关键作用. 本研究在水稻基因组中克隆到一个C2H2型锌指蛋白,命名为OsZAT12. 生物信息学分析显示OsZAT12全长597bp,其中不包含内含子,其蛋白质序列含有199个氨基酸,具有两个典型的C2H2锌指结构. OsZAT12蛋白定位于细胞核,在根中特异性表达.过表达拟南芥植株与野生型比较,其根变短,子叶的变绿率明显降低,植株矮小.初步推断OsZAT12可能影响植物生长发育尤其是根的伸长.

     

    Abstract: Abstract: Zinc finger proteins are a large family of plant transcriptional regulatory factors that play a key role in plant development and environmental stress through transcriptional regulation, post-translational regulation, and protein-protein interactions. In this study, a C2H2-type zinc finger protein was cloned in the rice genome and named as OsZAT12. Bioinformatics analysis showed that OsZAT12 is 597bp with full-length which does not include introns, and its protein sequence contains 199 amino acids with two typical C2H2 zinc finger structures. OsZAT12 is localized in the nucleus and is specifically expressed in roots. Compared with wild type, the root length of overexpressed Arabidopsis is shortened, the greening rate of cotyledons is significantly lower, and the plant is dwarfish. Therefore, it preliminary implied that OsZAT12 may be involved in the growth and development of plant, especially root elongation.

     

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