Abstract: A ratiometric fluorescence probe based on 8-Aminoquinoline for Zn2+ was designed and synthesis. The structure and characterization were researched by 1HNMR, MS, UV-vis absorption spectrum and fluorescence spectrum. The experiment results indicated that the probe showed good recognition for Zn2+ under pH for 4-9.5 conditions, especially in the pH 7.4, the physiological condition. The probe can combine Zn2+ to a stable complex at the ratio of 1:1. The fluorescence probe showed high sensitivity, excellent selectivity and fast fluorescence response toward Zn2+. Therefore, the probe had the potential to recognize Zn2+ in the fields of life and environment.