Abstract: As a new type of artificial periodic dielectric nanostructure, photonic crystals will have extensive applications in the future. Holography lithography which synthesizes nanostructure based on multi-beam interference principle, is one of the most promising fabricate method of photonic crystal structure. Different optical parameters will influence the photonic crystal structure and using experimental research to conclude its law is inefficient. However, we can theoretically get the light intensity distribution of interference pattern using the numerical simulation. Therefore, study the influence of optical parameters using computer simulation is necessary. In this paper, we study the two dimensional photonic crystal structure formed by multi-beam interference using Matlab simulation. The influence on interference pattern of optical parameters like polarizing angle, phase shift, azimuth angle and polar angle was studied by simulation. According to the result, the azimuth angle affect the symmetries of interference pattern; the change of polarizing angle leads to the change of absolute position and maximum and minimum value of interference light intensity; the phase shift has less influence on interference structure, but it should be considered during the fabrication due to its influence on accuracy of position; the polar angle will influence the spatial distribution of light intensity. This work will support getting the optical parameters of special photonic crystal structure using intelligent algorithms.