响应面法优化酶解鱼鳞制备ACE抑制肽的研究

曾健辉, 朱宝君, 王娟, 孔繁茂, 马广智, 黄儒强

曾健辉, 朱宝君, 王娟, 孔繁茂, 马广智, 黄儒强. 响应面法优化酶解鱼鳞制备ACE抑制肽的研究[J]. 华南师范大学学报(自然科学版), 2016, 48(4): 57-61. DOI: 10.6054/j.jscnun.2016.06.001
引用本文: 曾健辉, 朱宝君, 王娟, 孔繁茂, 马广智, 黄儒强. 响应面法优化酶解鱼鳞制备ACE抑制肽的研究[J]. 华南师范大学学报(自然科学版), 2016, 48(4): 57-61. DOI: 10.6054/j.jscnun.2016.06.001
ZENG Jianhui, ZHU Baojun, WANG Juan, KONG Fanmao, MA Guangzhi, HUANG Ruqiang. Optimization of Enzymatic Hydrolysis Preparation of ACE Inhibitory Peptides from Fish Scale by Response Surface Method[J]. Journal of South China Normal University (Natural Science Edition), 2016, 48(4): 57-61. DOI: 10.6054/j.jscnun.2016.06.001
Citation: ZENG Jianhui, ZHU Baojun, WANG Juan, KONG Fanmao, MA Guangzhi, HUANG Ruqiang. Optimization of Enzymatic Hydrolysis Preparation of ACE Inhibitory Peptides from Fish Scale by Response Surface Method[J]. Journal of South China Normal University (Natural Science Edition), 2016, 48(4): 57-61. DOI: 10.6054/j.jscnun.2016.06.001

响应面法优化酶解鱼鳞制备ACE抑制肽的研究

基金项目: 

广东省产学研项目(2013B090600153);广东省科技计划项目(2013B020312004);广东省海洋渔业科技推广专项科技攻关项目(A201301C10)

详细信息
    作者简介:

    马广智,教授,Email:magzh59@126.com;黄儒强,教授级高级工程师,Email:qiangdoctor@126.com.

    马广智,教授,Email:magzh59@126.com;黄儒强,教授级高级工程师,Email:qiangdoctor@126.com.

    通讯作者:

    马广智,教授,Email:magzh59@126.com;黄儒强,教授级高级工程师,Email:qiangdoctor@126.com.

    马广智,教授,Email:magzh59@126.com;黄儒强,教授级高级工程师,Email:qiangdoctor@126.com.

  • 中图分类号: TS254.9

Optimization of Enzymatic Hydrolysis Preparation of ACE Inhibitory Peptides from Fish Scale by Response Surface Method

  • 摘要: 鱼鳞是鱼类加工的下脚料之一,含有丰富的蛋白质和矿质元素等营养物质,是非常好的可利用生物资源. 文中研究了碱性蛋白酶酶解鱼鳞制备ACE抑制肽的优化工艺. 以ACE抑制率为指标,在酶解温度、酶解pH、加酶量、底物质量浓度等条件下先进行单因素实验,在此基础上运用响应面法优化碱性蛋白酶酶解鱼鳞制备ACE抑制肽的工艺条件. 结果表明:在加酶量61%(约12 000 U/g)、pH 89、温度547 ℃的条件下酶解2 h,ACE抑制率理论值为8536%,实际酶解物的ACE抑制率为862%,相对误差为091%.
    Abstract: Fish scale is one of the fish processing leftover, with abundant nutrients like protein and mineral elements, which makes it a usable biological resource. This paper study the preparation of ACE inhibitory peptides by alkaline protease hydrolysis of the fish scale. The enzymatic hydrolysis conditions for the preparation of ACE inhibitory peptides were optimized using the response surface method based on single factor experiments, including hydrolysis temperature, hydrolysis pH, enzyme dose and substrate concentration. The results showed that the optimal enzymatic hydrolysis conditions were as follows: enzyme dose 61% (about 12 000 U/g), pH 89, hydrolysis temperature 547 ℃ for 2 hours. Under these conditions the theoretical value of ACE inhibition rate was 8536%, and the actual ACE inhibition rate was 862%, in which the relative error was 091%.
  • 期刊类型引用(4)

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    2. 李路瑶,曾丽芳,宋益善,陈建康. 草鱼鳞酶解物的制备及其促嗜热链球菌生长作用. 上海海洋大学学报. 2021(06): 1132-1141 . 百度学术
    3. 涂丹,张益奇,林亚楠,叶繁,戴志远. 热处理对鱼鳞蛋白酶解特性的影响. 中国食品学报. 2020(03): 149-155 . 百度学术
    4. 涂丹,张益奇,叶繁,戴志远. 酶解制备鱼鳞蛋白降血压肽的工艺优化. 核农学报. 2019(01): 120-128 . 百度学术

    其他类型引用(3)

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出版历程
  • 收稿日期:  2015-12-31
  • 刊出日期:  2016-07-24

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