Optimization of Enzymatic Hydrolysis Preparation of ACE Inhibitory Peptides from Fish Scale by Response Surface Method
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摘要: 鱼鳞是鱼类加工的下脚料之一,含有丰富的蛋白质和矿质元素等营养物质,是非常好的可利用生物资源. 文中研究了碱性蛋白酶酶解鱼鳞制备ACE抑制肽的优化工艺. 以ACE抑制率为指标,在酶解温度、酶解pH、加酶量、底物质量浓度等条件下先进行单因素实验,在此基础上运用响应面法优化碱性蛋白酶酶解鱼鳞制备ACE抑制肽的工艺条件. 结果表明:在加酶量61%(约12 000 U/g)、pH 89、温度547 ℃的条件下酶解2 h,ACE抑制率理论值为8536%,实际酶解物的ACE抑制率为862%,相对误差为091%.Abstract: Fish scale is one of the fish processing leftover, with abundant nutrients like protein and mineral elements, which makes it a usable biological resource. This paper study the preparation of ACE inhibitory peptides by alkaline protease hydrolysis of the fish scale. The enzymatic hydrolysis conditions for the preparation of ACE inhibitory peptides were optimized using the response surface method based on single factor experiments, including hydrolysis temperature, hydrolysis pH, enzyme dose and substrate concentration. The results showed that the optimal enzymatic hydrolysis conditions were as follows: enzyme dose 61% (about 12 000 U/g), pH 89, hydrolysis temperature 547 ℃ for 2 hours. Under these conditions the theoretical value of ACE inhibition rate was 8536%, and the actual ACE inhibition rate was 862%, in which the relative error was 091%.
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Keywords:
- response surface method /
- enzymatic hydrolysis /
- fish scals /
- ACE inhibitory peptides
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期刊类型引用(4)
1. 苑园园,刘畅,王燚欣,宋晓鑫. 双酶水解鱼鳞蛋白制备ACE抑制肽的工艺优化研究. 现代农村科技. 2022(01): 77-79 . 百度学术
2. 李路瑶,曾丽芳,宋益善,陈建康. 草鱼鳞酶解物的制备及其促嗜热链球菌生长作用. 上海海洋大学学报. 2021(06): 1132-1141 . 百度学术
3. 涂丹,张益奇,林亚楠,叶繁,戴志远. 热处理对鱼鳞蛋白酶解特性的影响. 中国食品学报. 2020(03): 149-155 . 百度学术
4. 涂丹,张益奇,叶繁,戴志远. 酶解制备鱼鳞蛋白降血压肽的工艺优化. 核农学报. 2019(01): 120-128 . 百度学术
其他类型引用(3)
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