Abstract: To evaluate the rural human settlement in Karst landform region of Guizhou Province, three typical areas, Hongfeng community at Qinzhen city, Yachi community at Bijie city and Huajiang community at the junction of Zhenfeng county and Guanling county are selected as the examples. Firstly, assessment index system for actual environmental conditions in Karst is established on the basis of the existing theory and practice in human settlement evaluation. Then the statistic characters about socio-economic survey data of 29 administrative villages and the structure of ANN is combined to build and train a reasonable neural network. Lastly, the BP ANN is used to assess them. Results show that Hongfeng had better human settlement communities, most of Yachi and Huajiang resident level is in the mid or lower grade, and the worst assessment is in Huanjiang. Therefore, rural living environment in Karst area closely related to regional geographic environment, especially natural conditions, and it is still the key for Karst human settlement.